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SEC of a mixture containing protective antigen and 1G3 or 1G3 F(ab′)2 on a Superose 6 10/30 column. Supercomplexes that included 1G3 or 1G3-F(ab′)2 eluted at 8.3 and 9.0 mL, respectively, indicating the reduction in molecular mass upon cleavage of the antibody. The void volume corresponds to 6.0 mL.
