Fig. 3.
Differential expression of selected proteins in prostate from WT versus KI mice. (A) Serum starved WT and KI MEFs were serum-stimulated for 2 h and cell lysates were resolved by SDS/PAGE followed by Western blotting with the indicated antibodies. (B) DLP extracts were resolved by SDS/PAGE and Western blotting with the indicated antibodies was performed. (C–K) Sections of the prostate from WT, WT/cPtenF/F, and KI/cPtenF/F mice were used for IHC with the indicated antibodies. VP sections were used for detection of SMA and MMP3. CCL2 was detected in the anterior prostate. (Scale bar: 50 μm.)