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. 2010 Aug 9;190(3):307–315. doi: 10.1083/jcb.201005018

Figure 1.

Figure 1.

Localization of SHIP2 at CCPs. (A and B) TIRFM images of COS-7 cells expressing GFP-SHIP2 and either clathrin-mRFP (A) or mRFP-epsin (B). (A) Insets show the boxed areas at high magnification. White arrows point to SHIP2-positive CCPs, whereas red arrowheads point to SHIP2-negative CCPs. (C and D) Snapshots of clathrin-mRFP and GFP-SHIP2 fluorescence at a single CCP (C) and mean time course of relative fluorescence intensity at CCPs (D). (D) Error bars show mean ± SD. (E) Snapshots of GFP-SHIP2 and cortactin-DsRed fluorescence at a CCP. Bars: (A and B) 5 µm; (C and E) 1 µm.

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