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. 2010 Aug 9;190(3):307–315. doi: 10.1083/jcb.201005018

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© 2010 Nakatsu et al.

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Figure 4. — PI(3,4,5)P₃ production accelerates endocytic CCP dynamics. (A) Ratio of PI(3,4,5)P₃ to total inositol phospholipids (PIs) in control and SHIP2 KD cells after serum stimulation as determined by HPLC. Error bars show mean ± SD (*, P < 0.05; t test). (B) Schematic representation of the rapamycin-inducible heterodimerization system used to recruit a PI 3-kinase at the plasma membrane. (C) COS-7 cells expressing RFP–FKBP-iSH2 or GFP-Akt-PH in the absence or presence of the rapamycin analogue (RAPA). (D) Cumulative histograms of CCP lifetime in cells expressing RFP-FKBP (n = 186; blue), RFP–FKBP-iSH2 (n = 227; red), or RFP–FKBP-iSH2 in ruffle (n = 30; green). Bar, 10 µm.