Skip to main content
. Author manuscript; available in PMC: 2010 Aug 17.
Published in final edited form as: Traffic. 2008 Aug 6;9(10):1653–1664. doi: 10.1111/j.1600-0854.2008.00789.x

Figure 6.

Figure 6

(A) Compensating mutations of the S. castellii Nvj1 ortholog. The N-terminal hydrophobic core sequence and flanking positive charges of the S. castellii ortholog can be altered to confer strict perinuclear localization in S. cerevisiae. EGFP reporters were localized were localized in nvj1Δvac8Δ and nvj1ΔVAC8+ cells. Nuclear chromatin (blue) and vacuole membranes (red) were co-stained with Hoechst and FM4-64, respectively. Interactions between Nvj1p-EGFP and Vac8p on FM4-64 stained vacuoles create NV junctions, which appear yellow in the overlay. Arrowheads in vac8-Δ cells point to cortical ER localization. Arrowheads in VAC8+ cells point to extra-perinuclear junctions. (B) Expression of wt S. castellii Nvj1-EGFP in S. castellii cells. The S. castellii Nvj1 ortholog forms tight NV junctions in the presence of Vac8 and is evenly distributed around the nuclear envelope in vac8Δ cells. (C) Localization in K. lactis cells. S. cerevisiae Nvj1-EGFP strictly localizes to the perinuclear ER in K. lactis cells. K. lactis Nvj1-EGFP reporter does not localize exclusively to the perinuclear ER in K. lactis. The arrowhead indicates extra-perinuclear localization in K. lactis cells expressing the K. lactis Nvj1-EGFP ortholog.