Figure 4.
RGC axon terminals establish synapses with dendritic spines. A – D: YFP-immunopositive presynaptic profiles (pink) make synapses with dendritic spines (blue). In all panels, YFP-immunopositive, silver-enhanced gold particles are marked by the arrows. A: In this sample, an axon terminal makes contact with the spine neck. B: A spine receives multisynaptic input; a contact is made by a YFP-positive RGC axon terminal that itself synapses on other dendritic processes. C,D: Examples of YFP-identified RGC axon terminals (pink) that make synaptic contact with dendritic spines (blue). Scale bars = 200 nm.