TABLE 1.
Primary Antibody Information
| Antibody | Immunogen | Source/species in which raised/catalog No. |
Dilution |
|---|---|---|---|
| TrkB (Xenopus, full-length) |
Synthetic peptide; amino acid residues 396–411 of the cytoplasmic domain of Xenopus TrkB (LQNLSKASPVYLDILG) |
Bethyl Laboratories Inc. (Montgomery, TX) rabbit polyclonal custom-made |
1:2,000 |
| GFP | Green fluorescent protein purified from A. victoria |
Invitrogen (Eugene, OR) mouse monoclonal (IgG2a), clone 3E6 catalog No. A11120 |
1:10 |
| 3A10 mouse monoclonal antibody |
Generated in mice against chicken nervous tissue; recognizes a neurofilament-associated antigen in Xenopus RGCs (Hocking et al., 2008; Manitt et al., 2009) and spinal cord neurons (Gravagna et al., 2008) |
Developed by Drs. T. Jessell and J. Dodd (Columbia University), and obtained from the Developmental Studies Hybridoma Bank1 mouse monoclonal (IgG1) catalog No. 3A10 |
1:2,000 |
| SNAP-25 | Raised against synaptic vesicle- containing fractions immunoprecipitated from human brain homogenates using anti-human synaptophysin monoclonal antibodies |
Assay Designs (Ann Arbor, MI) mouse monoclonal (IgG1), clone SP12 catalog No. VAM-SV0012 |
1:1,000 |
| VAMP2 | Raised against a highly conserved peptide sequence of rat VAMP2 (residues 36–56) (QAQVDEVVDIMRVNVDKVLER) |
Assay Designs (Ann Arbor, MI) rabbit polyclonal catalog No. VAS-SV006 |
1:1,000 |
1
Developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA 52242.