Table 1.
Polymorphic STR markers for preimplantation PKHD1 haplotyping
| STR Name | STR Amplicon Location | Forward Primer (5′ --> 3′) | Reverse Primer (5′ --> 3′) |
|---|---|---|---|
| At the 5′ flanking region of PKHD1 gene: | |||
| 5–909 k | 52,969,730-52,970,172 | TGTGTCTGTGTAAATTAAAGTAGCACCA | GTTTCTTCTTTGTCTTCCTTCTCCGCTCT |
| 5–838 k | 52,899,197-52,899,465 | CTGGCCCAGTAACAATTTCGAG | GTTTCTTCTGTTAAAGCAAAGTTATCTTGAGGCAT |
| 5–570 k | 52,630,577-52,630,884 | TCCTCTTCTCTATTCAAAGGCAAC | GTTTCTTCACATTCTTGCCAGATTTGGTG |
| 5–564 k | 52,624,476-52,624,900 | TACAAAAAAAAATTAAAATTAGCTGGGCATCGT | GTTTCTTCACCATTTGGGAATAAGCCGAAG |
| 5–438 k | 52,498,725-52,499,021 | ATTTACAAGCCAAGAGAGACCTAGAACA | GTTTCTTCAATGTGGGGTTAAAATGGTAACTCTG |
| 5–326 k | 52,386,622-52,386,902 | GAAAGCTCCAGCCATCTAGCC | GTTTCTTAAGGGTTTGGTTTGTGTGAGTTAAGTCT |
| 5–85 k | 52,145,161-52,145,531 | AGTTGGGCATGGTGGCGTACAC | GTTTCTTGGTTGCAGCTTACTAATCATTGGATGTT |
| Within the PKHD1 gene: | |||
| 22 k | 52,037,793-52,038,039 | CCCAGCCCTTTGCCTTAGAATAG | GTTTCTTTATCACATGGAATGCCTAAAGCGAATTA |
| 28 k | 52,031,453-52,031,633 | TAGGTTGGTTGTTCCTTCTCTGAA | GTTTCTTGTTTTGAACTGGTCTGCCTTTTTAACAT |
| 72 k | 51,987,190-51,987,608 | AAATTATACCCTGTCTATTCGTCCG | GTTTCTTCACCAGTACCTGTCACAACACCT |
| 162 k | 51,898,142-51,898,312 | GCTCATCTACATTAAGGAAGGCAATCTT | GTTTCTTACTTGACTGGGCTATGGGGTAC |
| 329 k | 51,730,671-51,730,865 | CATTGTTAGGTGCTATGGGGTTTAG | GTTTCTTGGTGGGAGGATGGCTTAGGC |
| 387 k | 51,672,619-51,672,764 | ATACATCCTTAGAATGAAAAATTACTCAGGTA | GTTTCTTTATTATGCTGGGAATAGTATTAAATAGAACAA |
| At the 3′ flanking region of PKHD1 gene: | |||
| 3–104 k | 51,492,459-51,492,742 | TTGGGACCTGCTGGGCATAGTG | GTTTCTTGCTCTCCTGGTGTGTTAGATTGGATA |
| 3–101 k | 51,489,061-51,489,254 | TTCCTGGTTCTCCTTGTTTTTCTATCTT | GTTTCTTACCCTGGAGCAAGTAATCAATATCTGT |
| 3–85 k | 51,473,601-51,473,814 | GGCTTGATGGGGGAAGAGTATTG | GTTTCTTTTTTCTCATGTCTCTGCTGATGCTC |
| 3–32 k | 51,420,166-51,420,386 | GGAAAACATAATGAGTTAATCTCTGAATGC | GTTTCTTTCTCTCCCTGGATAATCTCTTTCATACT |
| 3–266 k | 51,354,413-51,354,612 | CCTATCTTCTTAGATTATTCTTGATTGTGCT | GTTTCTTCTGGATTATGCCTAGATAAAGGACTTTC |
| 3–263 k | 51,351,797-51,352,253 | ACTTAGCCAAACCTTTAAATAGATAG | GTTTCTTTTCATGAGATTTGTACCTGGCTTTAAGTTATT |
| 3–204.2 k | 51,292,282-51,292,464 | TCCTCAGAGTTCCACCTTGTGT | GTTTCTTGACATTATGAAAAGCAGACTTAGGCAAAT |