Table 1.

Work time, value of RNA concentration, purity and cycle threshold (Ct) resulting from Real-Time PCR. HPRT data are reported as endogenous Ct, GREM1 data are reported as target Ct since it was the gene expressed at the lower level

Extraction method	Work time	Total RNA (ng)	Abs 260/280	Abs 206/230	Total RNA (ng/μl)	Endogenous Ct	Target Ct
A	60 min	4,153 ± 1,025	1.24 ± 0.03	0.37 ± 0.02	207.65 ± 51.25	23.39 ± 2.05	28.44 ± 3.29
B	15 min	1283 ± 641	0.40 ± 0.08	0.06 ± 0.06	25.66 ± 12.82	35.86 ± 2.89	36.04 ± 3.27
C	30 min	124 ± 48	0.92 ± 0.05	0.11 ± 0.03	4.97 ± 1.92	22.43 ± 2.17	28.11 ± 3.17
D	30 min	103 ± 38	1.28 ± 0.03	0.08 ± 0.02	7.36 ± 2.72	20.94 ± 2.12	26.44 ± 3.16
E	30 min	61 ± 21	0.96 ± 0.04	0.07 ± 0.04	2.42 ± 0.84	27.73 ± 2.81	34.59 ± 3.77

Data are reported as mean±DS