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Plasma levels of nicotine and its metabolites in 92 male Japanese smokers 1 hour after smoking the first cigarette on the sampling day. Concentrations of nicotine (A), cotinine (B), and 3′-hydroxycotinine (C) were determined by LC/MS/MS. The sum (D) indicates the total concentration of nicotine, cotinine, and 3′-hydroxycotinine in each individual subject. Concentrations of 3′-hydroxycotinine were lower than the limit of detection in seven subjects.
