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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Future Virol. 2010 Jul 1;5(4):481–491. doi: 10.2217/FVL.10.33

Figure 1. Bimolecular complementation assay.

Figure 1

Human 293T cells were transfected with (A) N-terminal yellow fluorescent protein (NYFP)-tumor susceptibility gene (Tsg)101 alone, (B) C-terminal YFP (CYFP)-Ebola virus (EBOV) VP40 alone, (C) CYFP-Marburg virus VP40 alone, (D) NYFP-Tsg101 plus CYFP-EBOV VP40-ΔPT/PY, (E) NYFP-Tsg101 plus CYFP-EBOV VP40 or (F) NYFP-Tsg101 plus CYFP-Marburg virus VP40. Cells were examined at 24 h post-transfection for YFP fluorescence activity by confocal microscopy using a Zeiss LSM-510 Meta system.