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. 2010 Jul 28;30(30):10220–10232. doi: 10.1523/JNEUROSCI.1385-10.2010

Figure 7.

Figure 7.

Ghrelin stimulates OX2R-positive neurons in the BRASTO DMH and LH, but not in Sirt1-deficient mice. A, Serum levels of ghrelin in AL and DR wild-type mice (left) and in fed and fasted BRASTO (Tg) and control (WT) mice (right). Ghrelin levels are shown as mean values ± SEM (**p < 0.01, ***p < 0.001 by one-way ANOVA with Tukey–Kramer post hoc test, n = 5 for each condition). B, The number of cFOS-positive cells in the Arc, DMH, and LH of wild-type (WT) and BRASTO (Tg) mice at 90 min (left) and 120 min (right) after ghrelin injection (30 nmol/kg of body weight) and after PBS injection (right). cFOS-positive cells are shown as mean values ± SEM (*p < 0.05, **p < 0.01 by one-way ANOVA with Tukey–Kramer post hoc test for each hypothalamic nucleus, ghrelin injection, 90 min, n = 2–3 mice for each genotype, 7–12 sections per hypothalamic nucleus; 120 min, n = 3 for each genotype, 3–7 sections per hypothalamic nucleus, PBS injection, n = 2 for each genotype, 6–7 sections per hypothalamic nucleus). C, Rectal body temperature of BRASTO mice 120 min after ghrelin injection. Levels of rectal body temperature are shown as mean values ± SEM (*p < 0.05, **p < 0.01 by one-way ANOVA with Tukey–Kramer post hoc test, n = 6–7). D, Double immunofluorescent staining of cFOS and OX2R in the DMH and LH of wild-type (WT) and BRASTO (Tg) mice at 120 min after ghrelin injection. Arrows indicate cFOS/OX2R-double-positive cells. E, Quantification of the number of cFOS/OX2R-double-positive cells in the Arc, DMH and LH of wild-type (WT) and BRASTO (Tg) mice at 120 min after ghrelin injection. cFOS/OX2R-double-positive cells are shown as mean values ± SEM (*p < 0.05, n = 3 mice for each genotype, 3–7 sections per hypothalamic nucleus). F, Percentage of cFOS/OX2R-double-positive cells compared to a total number of cFOS-positive cells in the Arc, DMH, and LH of Sirt1+/+ and Sirt1−/− mice at 120 min after ghrelin injection. Percentages of cFOS/OX2R-double-positive cells are shown as mean values ± SEM (*p < 0.05, ***p < 0.001, n = 3 mice for each genotype, 6–12 sections per hypothalamic nucleus). G, A model for the SIRT1-mediated neurobehavioral adaptation in the hypothalamus in response to DR. See Discussion for details.