Fig. 1.

Activity of Eif4a3 translation-blocking morpholinos. Western blot analysis of exogenous (lanes 1-8) and endogenous (lanes 9 and 10) Eif4a3 protein, visualized using a mouse monoclonal anti-Eif4a3 antibody. Eif4a3MO inhibits translation of eif4a3 RNA containing the Eif4a3 morpholino binding site (5′UTR-4a3); translation of an RNA that lacks the Eif4a3MO binding site (4a3) is not inhibited. 21ng, 10.5ng, or 5.25ng Eif4a3 morpholino (MO) and/or 2ng 5′UTR-4a3 or 4a3 RNA were injected, as listed; higher morpholino doses did not lead to an appreciable increase in the degree of translational block (data not shown). Longer exposure times and 21ng morpholino (MO or 5 base pair mismatch, 5MM) were used for lanes 9 and 10. Whole-embryo lysates from stage 14 embryos were used for this study; β-Tubulin levels were used as a loading control.