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Eif4a3 is required for expression of tyrosinase genes. Whole-mount in situ hybridization with antisense probes against tyrosinase (tyr) (top panels) or tyrosinase-related protein-2 (dct) (bottom panels) in embryos injected with Eif4a3MO (4a3MO), Eif4a3MM (5MM), or Eif4a3MO+2ng eif4a3 RNA (Rescue). With both probes, intensity of signal and number of positive cells were reduced in Eif4a3 morphants at tailbud stages; this effect was rescued by co-expression of eif4a3 RNA.
