Skip to main content
. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: Dev Dyn. 2010 Jul;239(7):1977–1987. doi: 10.1002/dvdy.22330

Fig. 8.

Fig. 8

Apoptotic cell death is increased in Eif4a3 morphants. A: TUNEL assay of embryos injected with Eif4a3MO (4a3MO), Eif4a3MM (5MM), or Eif4a3MO+2ng eif4a3 RNA (Rescue) at stage 32. Strong staining observed in head only. B: Immunohistochemistry using Caspase 3 antibodies in embryos injected with Eif4a3MO (4a3MO), Eif4a3MM (5MM), or Eif4a3MO+2ng eif4a3 RNA (Rescue); signal was observed in the eyes of Eif4a3 morphants. C: Phosphohistone H3 antibody staining (blue) of embryos injected on one side (arrowhead) with Eif4a3MO (4a3MO) and 500pg β-gal RNA as a lineage trace (red) at stage 32. Phosphohistone H3 signal was not significantly affected by Eif4a3 knockdown.