Table I.

Effects of morpholino-mediated knockdown of EJC core components Eif4a3, Magoh, and Y14, scored at stage 32 (touch response) and stage 37 (melanophore and heart defects). nd=not determined. The Y14 morpholino was injected at lower concentration (2.6ng/embryo) than were the Eif4a3 or Magoh morpholinos: weak Y14 RNA-mediated rescue was achieved when 10ng/embryo or 20ng/embryo Y14 morpholino/embryo was used.

				Melanophore (%)
	Morpholino (ng/embryo)	RNA (ng/embryo)	Touch- responsive (%)	normal	reduced	none	Heart defect (%)	n
Eif4A3	10	0	0	13.3	55.6	31.1	88.9	46
	10	0.5	86.4	85.7	12.7	1.6	26.2	66
	10	2	91.1	82.1	14.3	3.6	14.8	56
	20	0	0	3.3	73.3	23.3	70	36
	20	0.5	94	78.8	17.3	3.8	34.6	50
	20	2	81	61.9	23.8	14.3	40	21
	0	2	78.9	52.6	34.2	13.2	23.7	38
Magoh	10	0	0	0	78.8	21.2	nd	83
	10	0.5	94.3	40	60	0	nd	31
	10	2	97.3	29.7	70.3	0	nd	74
	20	0	0	6	71.1	22.9	83.6	33
	20	0.5	100	64.5	35.5	0	nd	35
	20	2	98.6	75.7	21.6	2.7	10.2	37
	0	2	100	78.7	21.3	0	0	61
Y14	2.6	0	8.0	40.0	52.0	8.0	100.0	25
	2.6	0.5	100.0	91.9	8.1	0.0	2.7	37
	2.6	2	96.9	79.2	20.8	0.0	0.0	32
	0	2	82.6	72.7	13.6	13.6	34.8	23
uninjected			88.1	87.5	7.5	5.0	13.1	40