Table 5.
Aggregated phosphosite assignments (by site)
| Automated assignment |
||
|---|---|---|
| Pass | Fail | |
| All sites (total 129) | ||
| Manual assignment | ||
| Pass | 80 | 11 |
| Fail | 14 | 24 |
| Sites with two or more positive automatic assignments (total 60 sites versus 129) | ||
| Manual assignment | ||
| Pass | 52 | 39 |
| Fail | 8 | 30 |
| Sites with Rank 2 peptides or higher (109 sites) | ||
| Manual assignment | ||
| Pass | 72 | 13 |
| Fail | 8 | 14 |
| Sites with Rank 1 peptides (80 sites) | ||
| Manual assignment | ||
| Pass | 62 | 14 |
| Fail | 3 | 1 |
Each unique site is considered, taking all observations of that site. If any observation of a phosphosite is validated in any experiment then that site is called as validated.