Figure 1.—

Schematic overview of the promoter analysis vectors used in this study. The positions and relative orientations of the selectable marker gene hygromycin phosphotransferase (HPT) and the reporter gene EGFP-GUS are shown with respect to the right border (RB) and the left border (LB) of the T-DNA regions. The ccdB region was expected to be replaced by a promoter sequence by a LR reaction of the GATEWAY system. (A) The pHGWFS7 vector (Karimi et al. 2002). (B) pGYPSY harbors gypsy insulators (GY) at both ends of the EGFP-GUS expression cassette based on the pHGWFS7. (C) pGYPSY-TL with a translation leader (TL) inserted into pGYPSY. (D) pGYPSY-Su(Hw) with an overexpression cassette of Su(Hw), the binding protein of the gypsy insulator, inserted into pGYPSY. (E) pGYPSY-TL-Su(Hw) with a Su(Hw) overexpression cassette inserted into pGYPSY-TL.