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. Author manuscript; available in PMC: 2011 Aug 16.
Published in final edited form as: Immunol Lett. 2010 Jun 8;132(1-2):61–68. doi: 10.1016/j.imlet.2010.06.001

Figure 5. IL-2 deprivation creates prerequisite condition for TGF-β-mediated suppression by Tregs.

Figure 5

(A-B) Tconvs were stimulated by APCs and anti-CD3 mAb. In A, different amount of anti-IL-2 mAb or isotype control Ab was added into the cultures as indicated. In B, some cultures were added with TGF-β alone, or together with either anti-TGF-β mAb or isotype control Ab. (C-D) Tconvs isolated from wild type B6 (C) or IL-2−/− (D) mice were stimulated by B6 APCs and anti-CD3 mAb. Tconvs were cultured alone (Tconv group), or were co-cultured with Tregs from B6 mice either in the same chamber [Tconv:Treg (no transwell)] or in the different chambers [Tconv:Treg (transwell)] of a transwell. In C, cultures were treated with 10 μg/ml neutralizing anti-TGF-β mAb and/or 10 μg/ml anti-IL-2 mAb as indicated. In D, some cultures were treated with various concentration of anti-TGF-β mAb. Cell proliferation was assessed on day-3 by 3H-thymidine incorporation. Figures are representative of at least 3 independent experiments.