Skip to main content
. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Oncogene. 2010 Jan 25;29(16):2404–2414. doi: 10.1038/onc.2009.520

Figure 6.

Figure 6

The S305 residue in the K303R ERα mutant is a determinant of AIR. (a, b) MCF-7 WT, K303R and K303R S305A Arom P (a) and CHO WT, K303R and K303R S305A P (b) pools of stably transfected cells were plated in soft agar and then treated with AD (10nM) ± Ana (1μM) for 14 days. The number of colonies >50μm were quantified, and data shown are the mean colony number of two plates and representative of three independent experiments. Bars, SD. n.s.= nonsignificant, *P<0.01, **P<0.005. (c) MTT growth assay in MCF-7 Arom 1 and K303R Arom 1 cells treated for 6 days with AD (10nM), Ana (1μM) and/or the S305 peptide (0.6μg/well). Cell proliferation is expressed as fold change relative to AD-treated cells. The data are representative of three independent experiments, each performed in triplicate. Columns, mean. Bars, SD. n.s.=nonsignificant, *P=0.01 AD+Ana+S305 peptide compared to Ana+AD.