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. Author manuscript; available in PMC: 2010 Aug 17.
Published in final edited form as: Mol Biochem Parasitol. 2008 May 2;160(2):107–115. doi: 10.1016/j.molbiopara.2008.04.011

Fig. 1.

Fig. 1

PEXEL of PfHRPII is cleaved and N-acetylated. (A) Native PfHRPII purified from the saponin supernatant of trophozoite-infected RBCs was subjected to an Asp-N solution digestion and analyzed by MALDI-TOF MS. The inset Coomassie-stained gel (left) and anti-PfHRPII Western blot (right) confirms the purified protein. The peptide fragment at m/z 1953.87 (*) was selected for tandem MS analysis. (B) De novo sequencing of PfHRPII m/z 1953.87. CID analysis reveals that m/z 1953.87 is the most N-terminal peptide fragment and demonstrates the PEXEL in PfHRPII (bold and underlined) is cleaved after residue 47 (45RLL↓) and the mature N-terminus is N-acetylated (Ac-HE49). The absolute intensity of the highest signal for each spectrum is labeled in bold on the right vertical axis (2.4E+4 and 1675.1, respectively).