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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Bioorg Med Chem. 2010 Jun 22;18(16):6099–6108. doi: 10.1016/j.bmc.2010.06.053

Figure 1.

Figure 1

Schematic representation of the genetic system for selection of agents targeting protein-protein interactions. A pair of interacting proteins, designated as X and Y, are inserted downstream of genes coding for DNA-binding domains (DBD1 and DBD2) possessing orthogonal operator affinities (top). Upon induction of PTAC promoter with IPTG, the expressed fusions form a functional heterodimeric repressor that recognizes the corresponding chimeric O1–O2 operator embedded within the promoter region of the tricistronic HIS3-KanR-lacZ reporter operon (middle). Perturbation of the repressor complex with an agent selected from a library results in a relief of the promoter blockade and expression of the reporter genes (bottom).