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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: J Cell Biochem. 2010 Aug 1;110(5):1155–1164. doi: 10.1002/jcb.22630

Figure 5. Cholangiocyte apoptosis is decreased when mir-29-suppressing pathways are activated.

Figure 5

(A.) H69 cells were pre-treated overnight with recombinant human Sonic Hedgehog ligand (10 μg/mL) or LPS (1 μg/mL) followed by TRAIL treatment (4 ng/mL) for 8 hours. Nuclei were stained with DAPI and nuclei with apoptotic morphology were counted. The percent of cells with apoptotic nuclei is indicated (mean, +/− SEM). ** p < 0.01. (B.) H69 cells were transfected with GFP plus either pCDNA3.1 (empty vector) or pCDNA/myc 48 hours and again 24 hours prior to apoptosis counting (1:4 GFP:pCDNA plasmid ratio). Cells were then treated with TRAIL (1 ng/mL) for 8 hours, and GFP-positive cells were then assayed for apoptosis by DAPI staining (mean, +/− SEM). ** p < 0.01. A lower concentration of TRAIL was employed compared to panel A to account for some stress due to transfection.