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. Author manuscript; available in PMC: 2010 Aug 17.
Published in final edited form as: Neurosci Lett. 2008 Nov 5;449(3):252–257. doi: 10.1016/j.neulet.2008.10.101

Figure 1. Expression of endogenous eno2 in the retina.

Figure 1

RNA in situ hybridization was employed in order to analyze endogenous eno2 mRNA expression in the retina at 72 hpf (A–C) and 12 dpf (D–F)

A–C: Panel A shows a low power superior view of two whole mount 72hpf larvae (rostral left) that were hybridized with a cRNA probe specific to eno2 (upper larva) or a sense control probe (lower larva). Hybridized probe was detected using a histochemical reaction with a purple/blue reaction product. The region of each image encompassing the left eye is shown at higher magnification in panels B and C, as indicated by the dotted outline.

D–F: Panel D shows a low power view of two 15μm cryosections of eyes from 12dpf larvae that were hybridized with cRNA probe specific to eno2 (upper sample) or a control sense probe (lower sample). Hybridized probe was detected with a histochemical reaction producing a purple/blue product. Representative high magnification fields from similar sections are shown in panels E and F. Key: GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer