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. Author manuscript; available in PMC: 2010 Aug 17.
Published in final edited form as: Neurosci Lett. 2008 Nov 5;449(3):252–257. doi: 10.1016/j.neulet.2008.10.101

Figure 2. GFP expression in the retina of Tg(eno2:egfp)Pt404 zebrafish larvae.

Figure 2

A: The image shows a Z-plane projection of multiple confocal images of the head region of a live 5dpf Tg(eno2:egfp)Pt404 larva (rostral left, dorsal up) demonstrating GFP expression.

B: Single plane confocal image of GFP expression in the retina of a live 72hpf Tg(eno2:egfp)Pt404 larva.

C – F: Indirect immunofluorescence and confocal microscopy were employed to detect GFP expression in fixed 20μm thick retinal cryosections derived from Tg(eno2:egfp)Pt404 zebrafish at (C) 2dpf; (D) 3dpf; (E) 5dpf; and (F) 12dpf.

Key: R, retina; TeO, optic tectum; HB, hindbrain ON, optic nerve; L, lens; GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer.