Figure 3. Expression of GFP in retinal ganglion cells and their central projections in a Tg(eno2:egfp)^Pt404 zebrafish larva.

A single plane confocal micrograph is shown of a cranial oblique coronal cryosection derived from a Tg(eno2:egfp)^Pt404 zebrafish at 5dpf. Double indirect immunofluorescence was employed to detect GFP expression (green) and Zn8 immunoreactivity (red) simultaneously. Key: OT, optic tract; otherwise same as figures 1 and 2.