Table 1.
Membrane partition coefficient of anginex on liposomes
Liposome preparation | Membrane partition coefficient (SEM) |
---|---|
DOPC | 5.6×103 (1341) |
DOPS | 2.2×106 (289,589) |
DOPC: DOPS | 4.5×105 (164,198) |
DOPG | 3.2×106 (174,459) |
DOPC:DOPG | 7.9×105 (437,378) |
The binding of 10 μM anginex to lipid bilayers was assessed by measuring liposome-associated tryptophan fluorescence normalized for fluorescence intensity in the absence of liposomes. The membrane partition coefficient (K) was calculated according to the best fit of the data. Results are expressed as mean values (SEM) from at least two experiments with duplicate data points.