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. 2010 Jul 8;25(9):2374–2386. doi: 10.1093/humrep/deq123

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© The Author 2010. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited

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FISH analysis with the probes specific for chromosomes 13 (red), 15 (orange), 16 (aqua), 18 (pink), 21 (green) and 22 (yellow). (A) In the first polar body, there are two dots for each signal with the exception of chromosome 16 for which three signals are observed. (B) The corresponding oocyte was analyzed for the same chromosomes. As expected, as the first polar body is the mirror image of the chromosome status of MII oocytes, only one signal for chromosome 16 is detected in the oocytes, whereas two dots are present for the other tested chromosomes. Both images: ×600 magnification.