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. Author manuscript; available in PMC: 2010 Aug 17.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jun 11;878(23):2155–2162. doi: 10.1016/j.jchromb.2010.06.008

Figure 4.

Figure 4

Typical online column-switching LC-MS/MS SRM ion chromatograms for the determination of PhIP-C8-dG in (A) control calf thymus DNA (B) following the treatment of salmon testis DNA with 0.001 mg/mL of N-acetoxy-PhIP (C) colon DNA from mouse dosed with corn oil and (D) colon DNA from mouse treated with 50 mg/kg body weight of PhIP (50 μg of hydrolysed DNA was analysed (for (B) 10μg) and 1000 fmol of [13C10]PhIP-C8-dG internal standard on column). The SRM transitions monitored were m/z m/z 490 to 374 for PhIP-C8-dG and m/z 500 to 379 for [13C10]PhIP-C8-dG. The trap column was eluted using a gradient with solvent A, 0.1% formic acid and solvent B, acetonitrile (0.1% formic acid) at a flow rate of 120 μL/min. The analytical column was eluted isocratically with 0.1% formic acid/acetonitrile (0.1% formic acid) (75:25, v/v) at a flow rate of 120 μL/min.