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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Nat Immunol. 2010 Mar 14;11(4):303–312. doi: 10.1038/ni.1853

Figure 2.

Figure 2

Early activation of LN iNKT cells. (a-f) Animals were injected with α-GalCer or control particles and 3 days later LNs were analyzed. (a) Images of popliteal LNs from WT animals injected with control (top) or α-GalCer (bottom) particles. (b) Fold increase in total cell number (b) or in the depicted populations (c) in LNs of WT (b,c) and Jα18 (NKT KO, b) mice after injection with α-GalCer particles relative to animals injected with control particles; *p<0.05 (d) iNKT cells in mediastinal LNs after i.p. injection of control (left) or α-GalCer (right) particles (e,f) Percentage of iNKT cells in various LNs after i.p. (e) or s.c. foot-pad (f) injection of α-GalCer particles. +,draining; −,non-draining LNs. Each dot represents one LN, black lines represent mean. Data were compared with two-tailed t-test. (p**<0.005; ***p=0.0007). (g) CFSE-labelled adoptively transferred iNKT cells were detected as CFSE+CD1d-tet+TCRβ+B220−DAPI− two days after injection of α-GalCer (black) or control (grey) particles. (h-k) iNKT cell activation was measured as expression of CD25 (h-j) and CD69 (k) after i.p. injection of α-GalCer (black) or control particles (grey). MFI were normalized to mice injected with control particles. (l) NK1.1 expression in LN iNKT cells (left). Expression of CD25 (middle) and CD69 (right) in NK1.1+ (black) and NK1.1− (red) iNKT cells in mediastinal LNs 12 h after injection with α-GalCer (empty profiles) or control particles (filled profiles). (m) Intracellular IFN-γ staining for mediastinal LN-iNKT cells 12 h after injection of α-GalCer (black) or control (grey) particles.