Table 2.

Enzyme feed strategies for fed-batch simultaneous saccharification and co-fermentation of wheat straw (8%-11% WIS)^a

Time of addition (h)	Feed profile A (amount enzyme)	Feed profile B (amount enzyme)	Feed profile C (amount enzyme)	Feed profile D (amount enzyme)
Initial addition	2/6	1/5	1/5	1/5
6	1/6	1/10	-	-
12	1/6	1/10	-	-
18	1/6	1/10	-	-
24	1/6	1/10	1/5	1/5 (+ 2 g/L yeast)
32	-	-	1/5	1/5
36	-	1/5	-	-
40	-	-	1/5	1/5
48	-	1/5	1/5	1/5

^aIn all feed profiles, the same total enzyme load and the same substrate feed were used. Substrate was added after 6, 12, 18 and 24 h.