Table 2. The OX3860F insertion is linked to the male-determining locus.
Males: | 322 (52%) | Fluorescent: | 298 (93%) |
Wild Type: | 24 (7%) | ||
Females: | 302 (48%) | Fluorescent: | 29 (10%) |
Wild Type: | 273 (90%) |
The progeny from a cross between heterozygous OX3860F males (G6) and wild-type females shows that transgene transmission is highly skewed towards male progeny (93% of male progeny expressed the marker, versus only 10% of the female progeny, n = 624). The sex-ratio, however, is normal, indicating a male-linked insertion rather than female lethality. Non-parental phenotype was observed in 8.5% of the progeny, indicating a distance of 8.5 centiMorgans (cM) between the insertion and the male-determining locus.