Skip to main content
. 2010 Aug 17;5(8):e12181. doi: 10.1371/journal.pone.0012181

Figure 4. A 230 bp insertion in the proximal 3′ UTR of Comt is present in multiple strains.

Figure 4

PCR amplification of genomic DNA using primers flanking the 3′UTR (18,407,151 to 18,407,758 Mb) generates two different products. Strains containing the B2 SINE, such as B6, generate a larger PCR product (approximately 600 bp) then do strains lacking the SINE, such as D2 (approximately 400 bp). Genomic DNA from reciprocal hybrids (B6D2F1 and D2B6F1), which are heterozygous for the B2 SINE indel, generates both a short and long product due to inheritance of both B6 and D2 alleles, respectively.