Figure 1. Enrichment of cardiac specific phage by multiple rounds of biopanning.

After a single, screening cycle of phage incubated with H9C2 cells, recovered phage was amplified, tittered and injected intravenously into Balb/c mice. After a circulation time of 24 hours, mice were euthanized, heart and kidney dissected, digested with collagenase II, cells lysed and recovered phage tittered. Recovered phage was amplified, re-tittered and injected for subsequent round of biopanning. Phage recovered from heart versus kidney from each cycle of in vivo phage display was normalized by gram of tissue weight and expressed as a ratio of heart to kidney.