Figure 3. CTP functionally delivers the IKK/NF-kB inhibitory peptide NBD to cardiomyocytes.

H9C2 cells (a) and MCA205 (b) cells, were co-transfected with a NF-κB dependent luciferase reporter plasmid and a control renilla-expression plasmid. Twenty-four hours post-transfection, cells were stimulated with TNF-α (10 ng/ml) or pre-treated with increasing concentrations of CTP-NBD (50 uM, 100 uM, 200 uM and 400 uM) for 30 minutes followed by TNF-α stimulation for three hours. Cells were then washed, lysed and luciferase activity measured and normalized to renilla activity. (N = 4 in each group; error bars represent one standard deviation).