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nNOS immunoreactivity in locus coeruleus (LC) from a single pair used in the analysis, representing three separate anatomical levels: rostral (R), middle (M) and caudal (C). Each well was loaded with 10 μg of total protein. The bottom panel (right and left) shows immunoreactive actin probed with anti-actin antibody on the same blot as a control for protein loading and transfer.
