. Author manuscript; available in PMC: 2011 Sep 30.

Published in final edited form as: Virology. 2010 Jul 23;405(2):548–555. doi: 10.1016/j.virol.2010.06.017

Table 1.

Properties of MLV Envelope mutations

Env construct	Infectivity ^a		Syncytia ^b	Cell surface expression (FACS) ^c
Env construct	HIV-1 Core	MLV Core	Syncytia ^b	Cell surface expression (FACS) ^c
MLV Env	100	100	0±0	100
MLV/RD114	128±14	98±2	0.01±0.01	123
MLV Δ16	117±37	71±28	0.53±0.15	108
MLV Δ25	198±50	64±17	0.36±0.09	110
MLV Δ31	70±28	4±2	0.23±0.17	89
MLV Δ33	8±4	1±1	0±0	87
MLV R479G	0±0	0±0	0±0	51

I nfectivity values for each construct are shown as infectious units (IU) per ml relative to wildtype MLV Env (±SD, n=3).

The relative cell fusion index (CFI) was calculated per field by first determining the CFI relative to the CFI of each treatment group as described in materials and methods.

Truncations in the Env cytoplasmic tail fail to alter cell surface expression. Calculated as mean channel FL-2 fluorescence to mean channel GFP fluorescence for each construct relative to wildtype expression levels. Values shown at the average of two independent experimental replicates.