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. Author manuscript; available in PMC: 2011 Sep 30.
Published in final edited form as: Virology. 2010 Jul 3;405(2):289–299. doi: 10.1016/j.virol.2010.05.019

FIGURE 4.

FIGURE 4

Western blot of HPV L2 capsid protein expression with the L2-specific MAb H16.4B4. H16.4B4 monoclonal antibody was used to detect L2 protein expression from chimeric HPV infected raft tissues. H16.4B4 was raised against HPV18 L2 protein but cross-reacts with HPV16 L2 protein. Protein isolated from chimeric HPV18/CRPV virus infected raft tissues, expressing the CRPV L2 and L1 capsid proteins was used as a negative control. Protein isolated from wild-type HPV18 infected raft tissues was used as a positive control. Protein isolated from virus infected raft tissues were loaded, 90 or 120 μg to each well as labeled. Lanes 1–2: HPV18/CRPV infected tissues; Lanes 3–4: wild-type HPV18 infected tissues; Lanes 5–6: HPV18-L2(18)L1(18) infected tissues; Lanes 7–8: HPV18-L2(18)L1(16) infected tissues; Lanes 9–10: HPV18-L2(16)L1(18) infected tissues (cell line #1); Lanes 11–12: HPV18-L2(16)L1(18) infected tissues (cell line #2); and Lanes 13–14: HPV18-L2(16)L1(18) infected tissues (cell line #3). NOTE, that Lanes 9–14 represent three individually derived cell lines of the same mutant, HPV18-L2(16)L1(18). Molecular weight markers are on the left. The arrow indicates the position of the L2 protein.