FIGURE 7.
Infectivity assay of chimeric HPVs. Shown is a 2% agarose gel of nested RT-PCR-amplified HPV18 E1^E4 and ß-actin. All assays were done with use 1:20 dilution of the viral stock. Lane 1: negative control mock infected; Lane 2: positive control wildtype HPV18; Lane 3: HPV18-L2(16)L1(18/16); Lane 4: HPV18-L2(18)L1(18/16); Lane 5: HPV18-L2(18/16)L1(18); Lane 6: HPV18-L2(18)L1(16/18); Lane 7: HPV18-L2(16)L1(16/18); Lane 8: HPV18-L2(16/18)L1(18); Lane 9: HPV18-L2(16/18)L1(16); and Lane 10: HPV18-L2(18/16)L1(16). Positions of ß-actin and E1^E4 amplified sequences are shown on the right.