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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Int J Obes (Lond). 2010 Mar 16;34(9):1415–1426. doi: 10.1038/ijo.2010.51

Figure 7. Effect of switching C57BL/6 mice fed HFD to CD on adipocyte size and mammary gland development.

Figure 7

A. Representative H&E staining of mammary glands from BALB/c and C57BL/6 mice fed CD or HFD for 4 weeks; note significant increase in adipocyte size in C57BL/7 glands in HFD group. Scale bar, 60 μm. B. Representative H&E staining and mammary gland whole mounts from peripubertal C57BL/6 mice fed HFD for 4 weeks, then switched to CD or maintained on HFD for an additional 6 weeks; note decrease in adipocyte size and increase in duct length in C57BL/6 glands in group switched to CD. C. Representative H&E staining of mammary glands from CD- or HFD-fed OVX-C57BL/6 mice treated with saline (control) or estrogen. Note that E treatment for 5 days had no discernible effect on adipocyte size. H&E images scale bar, 60 μm. Whole mount images scale bar, 300 μm.