Fig. 3.

One-step growth kinetics of SeV mutants in Vero cells. Monolayer cultures of Vero cells were infected at an MOI of 3 CIU/cell with each mutant SeV. Vero cells were incubated with viruses for 1 h, then unabsorbed viruses were aspirated, cells were washed two times with PBS and fresh was added to each well. Supernatants were collected at 6, 24, 36, 48, 72, and 96 h p.i. and flash frozen. Plaque assays were performed on Vero cells and virus titers were determined for each time interval. * Zero titer indicates that virus titer at the indicated time point was below our detection threshold (50 CIU/ml). (A) SeV mutants with alanine substitutions in a DKDKD motif upstream of the glycine-rich motif. (B) SeV mutants that showed delayed growth in Vero cells or lower titers as compared to rWT. (C) SeV mutants with substitutions in and around the glycine-rich motif. The data represent the mean ± standard deviation of two independent experiments.