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. Author manuscript; available in PMC: 2011 Sep 30.
Published in final edited form as: Virology. 2010 Jul 3;405(2):269–279. doi: 10.1016/j.virol.2010.06.006

Figure 1.

Figure 1

HSV-2 2.12gE-del124–495gfp (gE2-del) construction and characterization. A. Diagram of 2.12gE-del124–495gfp. 5′ flanking sequence: the white box represents sequences prior to the ATG protein coding start site, while the black box indicates the first 369 bp of the gE-2 protein coding sequence. 3′ flanking sequence: the black box represents 156 bp of the gE-2 protein coding sequence prior to the stop codon, while the white box indicates the remaining 3′ flanking sequences. The green fluorescent protein under the control of a CMV promoter was inserted into Us8 to replace the 1,116 deleted bp. B. Western blot of Vero cells infected with gE2-del, rgE2-del or WT virus probed with antibodies to VP5, gE, gD, and Us9.