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Virus spread from neuron cell bodies along axons and to epithelial cells in Campenot chambers. A and B. Vero cells were added to the N chamber one day prior to infection. 105 PFU of WT, gE2-del, or rgE2-del virus was added to the S chamber and at 48 hpi the contents of the S (A) and N (B) chambers were titered on Vero cells. The limit of detection for the titration is 1 PFU.
