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. Author manuscript; available in PMC: 2011 Sep 30.
Published in final edited form as: Virology. 2010 Jul 3;405(2):269–279. doi: 10.1016/j.virol.2010.06.006

Figure 8.

Figure 8

Spread of virus from epithelial cells to neuron cell bodies in Campenot chambers. A and B. HaCaT cells were seeded in the N chamber and allowed to polarize for 1 week prior to infection with 1 × 105 PFU of WT, gE2-del, or rgE2-del virus. At 48 hpi, contents of the S (A) and N (B) chamber were harvested and titered on Vero cells. C. Neurites in the N chamber were infected with 1 × 105 PFU of WT, gE2-del, or rgE2-del virus in the absence of HaCaT cells. At 24 hpi, contents of the S chamber were harvested and titered on Vero cells. n=5 chambers for each assay ± SEM.