. 2010 May 6;117(1):4–16. doi: 10.1093/toxsci/kfq134

TABLE 1.

Matrix and Anticoagulant Affect on Cytokine Values Measured by Multiplex Immunoassay in Humans

	Higher^a	Equivalent^b	< 50% Reported^c
IL1α		C^d, H^d, S^d
IL1β	C^d; A^e		H^d; S^e
IL2	A^e	C^d, H^d, S^d	S^e
IL4	A^e, (H^e)		C^d, H^d, S^d
IL5	C^d, S^d; A^e, (H^e)	H^d, S^d	S^e
IL6	C^d; A^e	H^d, S^d, ^e
IL7	A^e, (H^e)
IL8	H^e, S^e	C^d, H^d, S^d, ^e
IL10	A^e, (H^e)	C^d, H^d, S^d
IL12	A^e, (H^e)	C^d, H^d, S^d
IL13	C^d; A^e, (H^e)		H^d, S^d
IFNγ	A^e, (H^e)	C^d, H^d, S^d
TNF-α	C^d; A^e, (H^e)		H^d, S^d
RANTES			C^d, H^d, S^d
Eotaxin	H^d		C^d, S^d

Note. A, acid citrate dextrose plasma; C, citrate plasma; H, lithium heparin plasma; S, serum.

Cytokines with geometric mean or median values (depending on study) that exceed by ≥ 40% the value in the next highest matrix. When the second highest value also exceeds by 40% the third matrix, this matrix is in parentheses. 40% chosen to exceed the analytical variability (except IL13 in Wong et al., 2008).

Cytokine geometric mean or median value (depending on study) is within 40% of the highest value for the cytokine.

Values not reported are beyond the working range of the assay, i.e., below the lower limit or above the upper limit of detection.

Hosnijeh et al. (2009).

Wong et al. (2008).