FIG. 2.
Diminished response of Fe pathway to Mn in HD model. Lysates harvested from STHdhQ7/Q7 (dark gray bars) or STHdhQ111/Q111 (light gray bars) cell lines after 30 h of Mn(II) chloride exposure versus vehicle control were analyzed by Western blot for TfR and actin. Representative blots showing three samples of each genotype-exposure group are shown, Mn(II) exposure as indicated. Quantification of TfR protein expression in striatal cell lines, bar graph plotted as mean levels (±SEM) normalized to actin as a percent of the untreated wild-type control (n = 7). Table shows statistical analysis of a two-factor univariate ANOVA for relative TfR protein levels, significant effects of genotype and Mn(II) exposure are designated by shaded numbering. “*” Indicates significant differences between the four genotype-treatment groups (p < 0.05, post hoc t-test).