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. 2010 Jun 16;117(1):180–189. doi: 10.1093/toxsci/kfq176

FIG. 5.

FIG. 5.

Representative immunoblots are presented for NF proteins (NFH, NFM, and NFH) and KIF5-HC in NF preparations from control and HD-intoxicated (400 and 175 mg/kg/day dose rates) rats. (A) Immunoblots derived from proteins separated in nondenaturing gel conditions (Blue native PAGE). Proteins were loaded on gels at 50 μg per lane. (B) Immunoblots derived from proteins separated in denaturing gel conditions (tris-acetate/SDS-PAGE). Gels for NF immunoblots from HD samples were loaded at 2.5–5.0 μg protein per lane, whereas proteins from control samples were loaded at 25 μg per lane. Gels for kinesin immunoblots were loaded at 5 μg regardless of sample origin (control vs. HD).