TABLE 1.
Experiment and irradiation dose | Hormonal suppression | Analysis time (weeks after irradiation) | Body weight (g) | Testis weight (mg) | SV weight (mg) | Serum testosterone concentration (ng/ml) |
Exp. 1 | ||||||
10.5 Gy | Sham | 11 | 29 ± 1a | 25 ± 1a | 300 ± 12a | 2.8 ± 0.8a |
11.5 Gy | 4 weeks GnRH-ant | 11 | 27 ± 1a | 24 ± 1a | 260 ± 7b | 3.2 ± 0.9a |
12.5 Gy | 11 weeks GnRH-ant | 11 | 25 ± 1b | 14 ± 1b | 22 ± 2c | 0.5 ± 0.1b |
Exp. 2 | Sham | 11 | 28 ± 0d | 29 ± 1d | 256 ± 12d | 1.2 ± 0.3d |
12 Gy | 4 weeks GnRH-ant + flutamide | 4 | 25 ± 0e | 14 ± 0e | 21 ± 3e | 2 ± 0.9d |
13.5 Gy | 4 weeks GnRH-ant + flutamide | 11 | 27 ± 0d | 27 ± 1d | 241 ± 7d | 0.9 ± 0.2d |
11 weeks GnRH-ant + flutamide | 11 | 25 ± 0e | 11 ± 0f | 10 ± 1e | 0.1 ± 0.0e |
Note. The data of three irradiation doses from Exp. 1 were pooled because ANOVA analysis showed no significant difference among them. The data from the two irradiation doses from Exp. 2 were pooled because t-test analysis showed no significant difference between them except for the SV weights of 11-week treatments (9 ± 0 mg at 12 Gy vs. 13 ± 2 mg at 13.5 Gy), both of which were dramatically lower than those in control mice. N was between 9 and 29 for weights and between 5 and 12 for serum testosterone concentrations. For a given parameter, values that are significantly different from each other (p < 0.05) within each experiment are indicated by different letters.