Fig. 3.

Palmitate synthesis. Palmitate is synthesised in bulk solution or in liposomes, based on the FAS-B catalysis, and analysed by TLC. The palmitate released is ¹⁴C-labeled by [2-¹⁴C] malonyl-CoA incorporation. The following samples are separated by TLC after synthesis: line 1, palmitate synthesis (p.s.) entrapped in liposomes supplying Proteinase K externally (sample applied twice more concentrated); line 2, p.s. encapsulated in liposomes and no Proteinase K added externally; line 3, p.s. in liposomes without FAS-B enzyme; line 4, p.s. in liposomes without acetyl-CoA; line 5, p.s. in bulk solution and no Proteinase K supplied; line 6, p.s. in bulk solution with Proteinase K; line 7, [2-¹⁴C] malonyl-CoA (0.74 nmoles). The arrow shows the position expected for palmitate as determined in a separate TLC (same conditions) experiment with non-radioactive palmitic acid standard (not shown)