Fig. 4.

FRET assay for vesicle membrane growth. a Curve of normalised F _don/F _acc versus aliquots of palmitate micelles added on preformed POPC vesicles. b number weighted DLS size distribution of preformed vesicles re-extruded after re-hydration, the DLS analysis was performed before the incubation with palmitate micelles, c curve of normalised F _don/F _acc versus time (hours) as result of palmitate synthesis in the inner water pool of liposomes and incorporation in boundary layer. FRET assay for vesicle with internal FAS catalysis and Proteinase K added externally (diamonds), without precursor malonyl-CoA (squares), with no FAS-B enzyme in reaction (triangles), d number weighted DLS size distribution of vesicles re-extruded after re-hydration of the freeze-dried vesicles with the FAS-B catalysis solution, the DLS analysis was performed before the incubation period. The error bars reported in the graphs represent the range of values of experiments performed in triplicate